Silylation reagents - BSTFA, SILYL-991
For improved volatility, better thermal stability or low limit of detection in gas chromatography prerequisite: quantitative, rapid and reproducible formation of only on derivative halogen atoms introduced by derivatisation (e.g. trifluoroacetates) allow specific detection (ECD) with the advantage of high sensitivity elution order and fragmentation patterns in MS can be influenced by a specific derivatisation.
Reagents for silylation - alkylation (methylation) - acylation are available.
N,O-bis-trimethylsilyl-trifluoroacetamide
m.w. 257.4, Bp 40°C (12mm Hg), density d20°/4° = 0.961
BSTFA: R = CF3 powerful trimethylsilyl donor with approximately the same donor strength as the non-fluorinated analogue BSA
advantage of BSTFA over BSA: greater volatility of its reaction products (particularly useful for GC of some lower boiling TMS amino acids.
BSTFA is nonpolar (less polar than MSTFA), and can be mixed with acetonitrile for improved solubility. For silylating fatty acid amides, hindered hydroxyls and other compounds, which are difficult to silylate (like secondary alcohols and amines), we recommend BSTFA + 1% trimethylchlorosilane (TMCS), available under the designation SILYL-991.
Tuotekoodi | Valmistaja | Nimi | Description | Capacity (ml) | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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4001486 | 701220.201 Macherey-Nagel GmbH & Co. KG |
Silylation reagent BSTFA pack of 20x1 ml 20 |
BSTFA | 1 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4001487 | 701220.510 Macherey-Nagel GmbH & Co. KG |
Silylation reagent BSTFA 5 |
BSTFA | 10 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4001509 | 701490.1100 Macherey-Nagel GmbH & Co. KG |
Silylation reagent Silyl-991 pack of 1x100 ml UN 1 |
SILYL-991 (BSTFA - TMCS (99:1) | 100 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4001510 | 701490.150 Macherey-Nagel GmbH & Co. KG |
Silylation reagent Silyl-991 pack of 1x50 ml 1 |
SILYL-991 (BSTFA - TMCS (99:1) | 50 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4001511 | 701490.201 Macherey-Nagel GmbH & Co. KG |
Silylation reagent Silyl-991 pack of 20x1 ml 20 |
SILYL-991 (BSTFA - TMCS (99:1) | 1 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6803320 | 701220.110 Macherey-Nagel GmbH & Co. KG |
Silylation agent BSTFA Chemistry - test kit UN 1 |
BSTFA | 10 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Due to their purpose, derivatisation reagents are very reactive substances. For this reason they should be stored cool and protected from moisture. The derivatisation reagents are supplied in vials with crimp caps for easy access with a syringe. Vials with pierced sealing disks have limited stability and should be used immediately. |